COVID-19 IgM/IgG Antibody Detection Kit

COVID-19 IgM/IgG Antibody Detection Kit

(Colloidal Gold Immunochromatography Method) Product Manual

【PZotsatira RODUCT NAME】 COVID-19 IgM/IgG Antibody Detection Kit (Njira ya Colloidal Gold Immunochromatography) 【PACKAGING Chithunzi cha SPECIFICATIONS】 1 Mayeso / Kit, Mayeso 10 / Kit

【ABSTRACT】

Ma coronaviruses atsopano ndi amtundu wa β. COVID-19 ndi matenda opatsirana pachimake. Nthawi zambiri anthu amavutika. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda; anthu omwe ali ndi matenda asymptomatic amathanso kukhala gwero lopatsirana. Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yobereketsa ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7. The mawonetseredwe aakulu monga malungo, kutopa ndi youma chifuwa. Kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka nthawi zingapo.

【EXPECTED USAGE】

Chidachi ndi choyenera kuzindikirika bwino kwa COVID-19 pozindikira ma antibodies a 2019-nCoV IgM/IgG mu seramu yamunthu, plasma, kapena magazi athunthu. Zizindikiro zodziwika bwino za matenda a 2019-nCoV ndi monga kupuma, kutentha thupi, chifuwa, kupuma movutikira, komanso dyspnea. Zikavuta kwambiri, matenda amatha kuyambitsa chibayo, matenda oopsa kwambiri, kulephera kwa impso, ngakhale kufa kumene. 2019 nCoV imatha kutulutsidwa kudzera m'madzi opumira kapena kufalikira kudzera m'madzi am'kamwa, kuyetsemula, kukhudza thupi, komanso kudzera m'malovu a mpweya.

【PRINCIPLES OF THE PMtengo ROCEDURE】

Mfundo ya immunochromatography ya zida izi: kulekanitsa zigawo zikuluzikulu mu osakaniza kudzera sing'anga ntchito capillary mphamvu ndi yeniyeni ndi mofulumira kumanga oteteza antigen ake antigen. Mayesowa ali ndi makaseti awiri, kaseti ya IgG ndi kaseti ya IgM.

Kwa YXI-CoV- IgM&IgG- 1 ndi YXI-CoV- IgM&IgG- 10:Mukaseti ya IgM, ndi sing'anga yowuma yomwe idakutidwa padera ndi 2019-nCoV recombinant antigen ("T" test line) ndi mbuzi anti-mbewa. ma antibodies a polyclonal ( "C" control line). Ma antibodies omwe ali ndi golide wa colloidal, mbewa anti-human IgM (mIgM) ali mu gawo lotulutsa. Ma antibodies a nCoV IgM ngati alipo, kupanga zovuta za mIgM-IgM. Chovuta cha mIgM-IgM chidzadutsa pa fyuluta ya nitrocellulose (NC fyuluta) kudzera mu capillary action. Ngati 2019-nCoV IgM antibody ilipo mu chitsanzo, mzere woyesera (T) udzakhala womangidwa ndi mIgM-IgM complex ndi kupanga mtundu. Ngati palibe antibody ya 2019-nCoV IgM mu zitsanzo, mIgM yaulere sidzamanga pamzere woyeserera (T) ndipo palibe mtundu womwe ungapangidwe. MIgM yaulere idzamanga pamzere wowongolera (C); mzere wowongolerawu uyenera kuwoneka pambuyo pa sitepe yodziwika chifukwa izi zimatsimikizira kuti zida zikugwira ntchito bwino.Mu kaseti ya IgG, ndi sing'anga youma yomwe idakutidwa padera ndi mbewa anti-human IgG ("T" test line) ndi Kalulu. antichicken IgY antibody ( "C" control line). Ma antibodies otchedwa colloidal golide, 2019-nCoV recombinant antigen ndi nkhuku IgY antibody ali mu gawo lotulutsa. Kamodzi kuchepetsedwa seramu, plasma, kapena magazi athunthu agwiritsidwa ntchito pagawo la pad (S), the

colloidalgold-2019-nCoV recombinant antigen idzamanga ma antibodies a 2019-nCoV IgG ngati alipo, ndikupanga colloidalgold-2019-nCoV recombinant antigen-IgG complex. Zovutazo zidzadutsa pa fyuluta ya nitrocellulose (NC fyuluta) kudzera mu capillary action. Ngati 2019-nCoV IgG antibody ilipo mu chitsanzo, mzere woyesera (T) udzakhala womangidwa ndi colloidalgold-2019-nCoV recombinant antigen-IgG complex ndi kupanga mtundu. Ngati palibe antigen ya 2019-nCoV IgG mu zitsanzo, antigen yaulere ya colloidalgold-2019-nCoV sidzamanga pamzere woyeserera (T) ndipo palibe mtundu womwe ungapangidwe. Antibody yaulere ya colloidal gold-chicken IgY idzamanga pamzere wowongolera (C); mzere wowongolera uwu uyenera kuwoneka pambuyo pa sitepe yodziwika chifukwa izi zimatsimikizira kuti zida zikugwira ntchito bwino.

Kwa YXI-CoV- IgM&IgG-02- 1 ndi YXI-CoV- IgM&IgG-02- 10: Mfundo ya immunochromatography ya zida izi: kulekanitsa zigawo mu osakaniza pogwiritsa ntchito sing'anga pogwiritsa ntchito capillary mphamvu komanso kumangirira kwachindunji komanso mwachangu kwa antibody ku antigen yake. The COVID-19 IgM/IgG Antibody Detection Kit ndi njira yoyeserera yodziwira matenda a IgG ndi IgM ku SARS-CoV-2 m'magazi athunthu, seramu kapena plasma. Mayesowa ali ndi zigawo ziwiri, gawo la IgG ndi gawo la IgM. Mu gawo la IgG, anti-anthu IgG yokutidwa m'chigawo cha mayeso a IgG. Pakuyesedwa, chitsanzocho chimakhudzidwa ndi tinthu tating'ono ta SARS-CoV-2 antigen-coated mu kaseti yoyesera. Kusakanizako kumasuntha motsatana ndi nembanemba chromatographically ndi capillary action ndikuchita ndi anti human IgG m'chigawo cha mayeso a IgG, ngati chitsanzocho chili ndi ma antibodies a IgG kupita ku SARSCoV-2. Mzere wachikuda udzawonekera mu gawo la mayeso a IgG chifukwa cha izi. Momwemonso, IgM yotsutsana ndi anthu imakutidwa m'chigawo cha mayeso a IgM ndipo ngati chitsanzocho chili ndi ma antibodies a IgM ku SARS-CoV-2, mawonekedwe a conjugate amakumana ndi anti human IgM. Mzere wachikuda umapezeka mugawo la mayeso a IgM chifukwa chake. Chifukwa chake, ngati chitsanzocho chili ndi ma antibodies a SARS-CoV-2 IgG, mzere wachikuda udzawonekera m'chigawo cha mayeso a IgG. Ngati chitsanzocho chili ndi ma antibodies a SARS-CoV-2 IgM, mzere wachikuda udzawonekera m'chigawo cha mayeso a IgM. Ngati chitsanzochi chilibe ma antibodies a SARS-CoV-2, palibe mzere wachikuda womwe udzawonekere m'gawo lililonse la mzere woyeserera, zomwe zikuwonetsa zotsatira zoyipa. Kuti ikhale yoyang'anira ndondomeko, mzere wachikuda udzawonekera nthawi zonse m'chigawo cha mzere wolamulira, kusonyeza kuti chiwerengero choyenera cha chitsanzo chawonjezeredwa ndipo kupukuta kwa membrane kwachitika.

【MAIN COMPONENTS】

Zindikirani: Zomwe zili mumagulu osiyanasiyana sizingasakanizidwe kapena kusinthidwa.

【MATERIALS TO BE PROVIDED BY USER】

•Pamba ya mowa

•Singano yotengera magazi

【STORAGE NDI EXPIRATIION】

Sungani zida pamalo ozizira komanso owuma pa 2 - 25 ° C.

Osaundana.

Zida zosungidwa bwino zimakhala zovomerezeka kwa miyezi 12.

【SAMPLE REQUIREMENTS】

Kuyesa ndi koyenera ku seramu yamunthu, plasma, kapena zitsanzo zamagazi athunthu. Zitsanzo ziyenera kugwiritsidwa ntchito mwamsanga mukatha kusonkhanitsa. Seramu ndi madzi a m'magazi: Seramu ndi madzi a m'magazi ziyenera kupatulidwa mwamsanga mukangotenga magazi kuti mupewe hemolysis.

【SAMPLE PRESERVATION】

Seramu ndi madzi a m'magazi ayenera kugwiritsidwa ntchito mwamsanga mutatha kusonkhanitsa ndikusungidwa pa 2-8 ° C kwa masiku 7 ngati sagwiritsidwa ntchito nthawi yomweyo. Ngati kusungirako kwa nthawi yayitali kumafunika, chonde sungani pa -20 °C kwa nthawi zosakwana miyezi iwiri. Pewani kuzizira mobwerezabwereza ndi kusungunuka.

Magazi athunthu kapena zotumphukira amayenera kuyesedwa pasanathe maola 8 mutatolera.

Zovuta za hemolysis ndi lipid magazi sizigwiritsidwa ntchito kuti zizindikire.

【TESTING METHOD】

Za YXI-CoV- IgM&IgG- 1 ndi YXI-CoV- IgM&IgG- 10:

Werengani malangizo mosamala musanagwiritse ntchito. Bweretsani Mzere Woyesera, chubu choyezera Chitsanzo, ndi zitsanzo ku kutentha kwa chipinda musanayese.

1. Onjezani 50 µl wamagazi Athunthu kapena otumphukira kapena 20 µl Seramu ndi madzi a m'magazi ku chubu chosungunula cha Zitsanzo ndikusakaniza bwino. Onjezani madontho 3-4 kugawo lachitsanzo.

2. Siyani kutentha kwa firiji kwa mphindi zisanu kuti muwone zotsatira zake. Zotsatira zoyezedwa pambuyo pa mphindi zisanu ndizolakwika ndipo ziyenera kutayidwa. Za YXI-CoV- IgM&IgG-02- 1 ndi YXI-CoV- IgM&IgG-02-10:

Werengani malangizo mosamala musanagwiritse ntchito. Bweretsani Mzere Woyesera, chubu choyezera Chitsanzo, ndi zitsanzo ku kutentha kwa chipinda musanayese.

1. Onjezani 25µl wamagazi athunthu kapena ozungulira kapena 10µl Seramu ndi madzi a m'magazi ku chubu chosungunula cha Zitsanzo ndikusakaniza bwino. Onjezerani madontho 4 ku pepala lachitsanzo

gawo.

2. Siyani kutentha kwa firiji kwa mphindi zisanu kuti muwone zotsatira zake. Zotsatira zoyezedwa pambuyo pa mphindi zisanu ndizolakwika ndipo ziyenera kutayidwa.

【[INTERPRETATION OF MAYESO RESULTS】