New Coronavirus(SARS-Cov-2) Nucleic Acid Detection Kit

New Coronavirus(SARS-Cov-2) Nucleic Acid Detection Kit

(Fluorescent RT-PCR Probe Method) Product Manual

【Product name 】New Coronavirus(SARS-Cov-2) Nucleic Acid Detection Kit(Fluorescent RT-PCR Probe Method)

【Packaging specifications 】25 Mayeso / Kit

【Intended uszaka】

Chidachi chimagwiritsidwa ntchito pozindikira zamtundu wa nucleic acid kuchokera ku coronavirus yatsopano mu nasopharyngeal swabs, oropharyngeal (pakhosi) swabs, anterior nasal swabs, mid-turbinate swabs, kutsuka m'mphuno ndi ma aspirates a m'mphuno kuchokera kwa anthu omwe akuwaganizira kuti ali ndi COVID19 ndi wothandizira zaumoyo. Kuzindikira kwa jini ya ORF1ab ndi N ya coronavirus yatsopano kutha kugwiritsidwa ntchito pozindikira matenda othandizira komanso kuyang'anira miliri ya matenda atsopano a coronavirus.

【Principles of the procedure 】

Zidazi zidapangidwira ma probes apadera a TaqMan ndi zoyambira zenizeni zopangidwira ma gene coronavirus (SARS-Cov-2) ORF1ab ndi ma gene a N. Njira ya PCR reaction ili ndi ma seti 3 a zoyambira zenizeni ndi zofufuzira za fulorosenti kuti azindikire zomwe akufuna, ndipo seti yowonjezereka ya zoyambira ndi zowunikira za fulorosenti zimagwiritsidwa ntchito ngati njira yoyendetsera mkati mwa zida zowunikira jini zakunyumba.

Mfundo yoyesera ndi yakuti kafukufuku wina wa fulorosenti amaphwanyidwa ndikuwonongeka ndi ntchito ya exonuclease ya Taq enzyme mu PCR reaction, kotero kuti gulu la mtolankhani fulorosenti ndi gulu lozimitsidwa fulorosenti amalekanitsidwa, kotero kuti fulorosenti monitoring dongosolo akhoza kulandira fulorosenti. chizindikiro, ndiyeno Kupyolera mu kukulitsa kwa PCR kukulitsa, chizindikiro cha fluorescence cha kafukufukuyo chimafika pamtengo wapatali-Ct mtengo (Cycle threshold). Pankhani ya palibe amplicon chandamale, gulu la mtolankhani la kafukufukuyu lili pafupi ndi gulu lozimitsa. Panthawiyi, kutulutsa mphamvu kwa fluorescence resonance kumachitika, ndipo fulorosenti ya gulu la mtolankhani imazimitsidwa ndi gulu lozimitsa, kotero kuti chizindikiro cha fulorosenti sichikhoza kudziwika ndi chida cha fulorosenti cha PCR.

Pofuna kuyang'anira kugwiritsidwa ntchito kwa ma reagents panthawi ya mayesero, zidazo zimakhala ndi zowongolera zabwino ndi zoipa: kuwongolera kwabwino kumakhala ndi malo omwe akuwongoleranso plasmid, ndipo kuwongolera koyipa ndi madzi osungunuka, omwe amagwiritsidwa ntchito poyang'anira kuipitsidwa kwa chilengedwe. Ndibwino kuti muyike kulamulira kwabwino ndi kulamulira koyipa panthawi imodzi poyesa.

【Main componets 】

Zindikirani:(1) Zomwe zili mumagulu osiyanasiyana sizingasakanizidwe kapena kusinthanitsa.

(2) Konzekerani regent yanu: zida za nucleic acid.

【Storage condizions ndi expiration date 】

For BST-SARS-25:Transport ndi sitolo pa -20 ± 5 ℃ kwa nthawi yaitali.

For BST-SARS-DR-25:Transport kutentha firiji. Sungani pa -20 ± 5 ℃ kwa nthawi yayitali.

Pewani kuzizira kobwerezabwereza. Nthawi yovomerezeka yakhazikitsidwa motsatira miyezi 12.

Onani chizindikiro cha tsiku lopangira ndi kugwiritsa ntchito.

Pambuyo kutsegula koyamba, reagent akhoza kusungidwa pa -20 ± 5 ° C kwa osapitirira 1 mwezi kapena mpaka mapeto a reagent nthawi, tsiku lililonse limabwera choyamba, kupewa mobwerezabwereza amaundana-thaw m'zinthu, ndi chiwerengero cha reagent amaundana. - thaw mkombero sayenera upambana 6 zina.

【Applicable instrument】ABI 7500, SLAN-96P, Roche-LightCycler-480.

【Sample requirements 】

1.Chitsanzo chachitsanzo chogwiritsidwa ntchito: Njira yothetsera nucleic acid.

2.Sample yosungirako ndi kayendedwe: Sungani pa-20 ± 5 ℃ kwa miyezi 6. Finyani ndi kusungunula zitsanzo zosaposa 6 nthawi.

【TEsting methodi】

1.Nucleic acid extraction

Sankhani zida zoyenera zochotsera ma nucleic acid kuti muchotse ma viral nucleic acid, ndikutsatira malangizo omwe ali nawo. Ndibwino kugwiritsa ntchito zida za Nucleic acid zochotsa ndi kuyeretsa zopangidwa ndi Yixin Bio-Tech (Guangzhou) Co., Ltd.

2.  Reaction reagent preparation

2.1 For BST-SARS-25:

(1) Chotsani SARS-Cov-2 Mix ndi Enzyme Mix, sungunulani kutentha kwa chipinda kusakaniza bwino ndi chipangizo cha Vortex ndiyeno centrifuge mwachidule.

(2) 16.5uL Enzyme Mix idawonjezeredwa ku 358.5uL SARS-Cov-2 Mix ndikusakaniza bwino kuti mupeze yankho losakanikirana.

(3) Konzani chubu choyera cha 0.2 mL PCR octal ndikuchiyika ndi 15uL ya njira yosakanikirana yomwe ili pamwambapa.

(4) Onjezani 15 μL ya yankho loyeretsedwa la nucleic acid, kuwongolera kwabwino ndi kuwongolera koyipa, ndikuphimba mosamala kapu ya octal chubu.

(5) Sakanizani bwino potembenuza mozondoka, ndipo mwachangu centrifuge kuti muyike madzi pansi pa chubu.

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