Nucleic Acid Extraction or Purification Kit
Nucleic Acid Extraction Or Kuyeretsedwa Kitkapena kusungidwa pa -20 ℃. Zitsanzo ziyenera kunyamulidwa pogwiritsa ntchito 0 ℃ curling.
Introduction
Nucleic Acid Extraction Or Purification Kit (Maginito Beads Method) adapangidwa kuti aziyeretsa zokha za RNA ndi DNA kuchokera kumadzi am'thupi (monga swabs, plasma, serum) pogwiritsa ntchito zida zopangira ma nucleic acid. Ukadaulo wa maginito a tinthu tating'onoting'ono umapereka DNA/RNA yapamwamba kwambiri yomwe ndiyoyenera kugwiritsidwa ntchito mwachindunji pamakina otsika monga kukulitsa kapena machitidwe ena a enzymatic.
Application Range
Magazi onse, plasma, seramu ndi zitsanzo za minofu zina zidapangidwa mwachindunji ndikugayidwa. Nucleic acid yomwe idatulutsidwa idasankhidwa mosankhidwa ndi mikanda ya maginito ya super paramagnetic nanometer. Ndiye zomanga thupi, zolengedwa mchere ayoni ndi organic zosafunika anachotsedwa ndi kutsuka solution.Finally, asidi nucleic anali eluted ndi eluent kupeza koyera nucleic asidi njira.
Kit Contents
| Mphaka. Ayi. | Chithunzi cha YXN-VIRAL01-32A-BR | Zigawo | ||
| -50A | - 100A | |||
| Kukula | 32Tes | 50 Mayeso | 100 Mayeso | |
| Lysis Buffer | 96 wellPre-pack ed Mbale 2 zidutswa | 25ml ku | 50 ml pa | Surfactant ndi Tris |
| Wash Buffer I | ★ 15 ml | ★ 30 ml | Mchere wambiri | |
| Sambani Buffer Il | ★6ml*2 | ★12ml*2 | Mchere wochepa kwambiri | |
| Elution Buffer | 10 ml pa | 20 ml pa | Mchere wochepa kwambiri | |
| MagaBio Reagent | 1.0ml pa | 2.0ml ku | Magnetic particles | |
| Kabuku(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Nakuti:ZaYXN-VIRAL01-32A-BR-50A,onjezani 15mL Mtheradi wa ethanol ku ★15mL Sambani Buffer I musanagwiritse ntchito; onjezani 24mL Mtheradi ethanol ku ★6mL Sambani bafa Il musanagwiritse ntchito. | ||||
| ZaYXN-VIRAL01-32A-BR-100A, onjezani 30mL Mtheradi wa ethanol ku ★30mL Sambani Buffer I musanagwiritse ntchito; onjezani 48mL Absolute ethanol ku ★12mL Sambani buffer Il musanagwiritse ntchito.【Zomwe zikuyenera kukonzedwa ndi wogwiritsa ntchito】Chonde konzani ethanol (gawo lowerengera) nokha. | ||||
Stlalanje Condizions
Pakafika zida, zida za zida zitha kusungidwa kutentha (15 - 25 ° C). Ma reagents ndi okhazikika mpaka chaka chimodzi kuyambira tsiku lopanga.
Sample Requirements
1. Zitsanzo zothandiza: Swabs, plasma, seramu ndi magazi athunthu etc.
2. Zitsanzo zosungira ndi zoyendetsa: Chitsanzocho chiyenera kuyesedwa mwamsanga
Materials ndi Devices Required but Not Provided
1. Magolovesi opanda ufa otayidwa
2. Chidebe cha Biohazard
3. Pensulo kapena pa
Procedure
Zotsatirazi zimagwiritsa ntchito mafuta odzola am'zigawo monga chitsanzo kuti afotokoze mwachidule masitepe opangira zida zopangira zida za nucleic acid m'zigawo.Bioer NPA-32 pkapenaSMART 32. Kwa mitundu ina ya zitsanzo, chonde onani buku la ogwiritsa ntchito. Itha kugwiritsidwanso ntchito ndi makasitomala malinga ndi zomwe adapeza:
1. Reagent Kukonzekera
a. ZaYXN-VB03-32A-50A ndi YXN-VB03-32A-100A
Onjezani 500uL Lysis Buffer ku gawo 1 ndi 7 la mbale ya 2.2mL 96-yakuya, 500uL Wash Buffer I ku gawo 2 ndi 8, 500uL Wash Buffer II ku ndime 3, 4 ndi 9,10; 70uL Elution Buffer ku gawo 5 ndi 11, 180uL madzi oyera ndi 20uL MagaBio Reagent ku gawo 6 ndi 12 (mikanda ya maginito iyenera kusakanizidwa bwino musanagwiritse ntchito),
b. ZaYXN-VB03-32A
Ikani ma reagents 96 odzaza bwino ndi kutentha. Gwirani mbale ya chitsime cha 96 mozondoka katatu, ndikudula thumba la pulasitiki. Centrifuge ndi chisanadze odzaza reagent kwa masekondi angapo (kapena kugwedezeka ndi dzanja kangapo) kupewa reagent kumamatira khoma la machubu. Chotsani chojambula cha aluminiyamu cha mbale ya 96-chitsime ndikuzindikira komwe mbaleyo ikulowera (mikanda ya maginito mu ndime #6 ndi #12),
2.Chitsanzo M'zigawo
1. Onjezani zitsanzo za 300uL pamizere 96 ya chitsime #1 ndi #7, chonde pewani kuipitsidwa,
2. Ikani mbale yakuya 96 pachidacho, ikani nsonga za mizere 8 pa chidacho,
3. Yambitsani pulogalamuyi motsatira njira zotsatirazi,
4. Pambuyo kuyeretsedwa basi kutha, kusamutsa elution Buffer mu mizati 5 ndi 11 kwa woyera antinuclear 0.5mL centrifuge chubu; Ngati simugwiritsa ntchito nthawi yomweyo, chonde sungani pa -20 ° C.
Performance Characteristics
1. Zomwe zimachotsedwa zimazindikiridwa ndi regent yodziwika bwino ya HBV DNA kuti ifike kukhudzidwa kwa 10 IU / mL. The yotengedwa mankhwala wapezeka ndi mkulu-tcheru HCV RNA kuzindikira reagent kufika tilinazo 50 IU/mL.
2. Sankhani zitsanzo za 4 (serum / plasma sample, nasopharyngeal swab sample, cervical exfoliated cell sample), chitsanzo chilichonse chimachepetsedwa nthawi za 10 ndi 3 gradients (kuphatikizapo chitsanzo choyambirira cha chiwerengero cha 4), pogwiritsa ntchito ma reagents oyenerera ndi oyesa mayeso kuti azindikire zamkati. jini yofotokozera molingana ndi malangizo azinthu, ndipo mtengo wa Ct wa batch iliyonse umasiyana ndi zosakwana 1.
| Step | Chabwino Malo | Program Dzina | Waizing Time(min:SS) | Mixndi Time(mmu:SS) | Magnet Time(min:SS) | Adsorption | Speed | Volume mphamvu(μL) | Tmlengalenga |
| 1 | 1 | Lysis | 0:0 ku0 | 2:00 pa | 0:0 ku0 | F | 700 | 80 | |
| 2 | 6 | Badzi | 0:0 ku0 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:0 ku0 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wphulusa1 | 0:0 ku0 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wphulusa2 | 0:0 ku0 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wphulusa3 | 0:0 ku0 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 pa | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Taya | 0:0 ku0 | 0:15 | 0:0 ku0 | F | 200 |
Safety
1. GENERAL CHITETEZO.
Kugwiritsa ntchito mankhwalawa m'njira yomwe sinafotokozedwe m'mabuku a ogwiritsa ntchito kungayambitse kuvulala kapena kuwonongeka kwa chida kapena chipangizocho. Onetsetsani kuti aliyense wogwiritsa ntchito mankhwalawa walandira malangizo okhudza chitetezo m'ma labotale komanso zambiri zachitetezo zomwe zaperekedwa m'chikalatachi.
1.1 Musanagwiritse ntchito chida kapena chipangizo, werengani ndikumvetsetsa zidziwitso zachitetezo zomwe zaperekedwa muzolemba za ogwiritsa ntchito zoperekedwa ndi wopanga chidacho.
1.2 Musanagwire mankhwala, werengani ndi kumvetsetsa Mapepala Onse Otetezedwa Otetezedwa (SDSs) ndikugwiritsa ntchito zida zoyenera zodzitetezera (magolovesi, mikanjo, chitetezo cha maso, ndi zina zotero). Kuti mupeze ma SDS, onani gawo la "Documentation and Support" m'chikalatachi.
2. Chemical chitetezo
WOGWIRITSA NTCHITO CHEMICAL. Kuti muchepetse zoopsa, onetsetsani kuti ogwira ntchito m'ma labotale amawerenga ndikutsata malangizo achitetezo okhudzana ndi kugwiritsa ntchito mankhwala, kusungirako, ndi zinyalala zomwe zaperekedwa pansipa, ndipo funsani ma SDS oyenera kuti musamalire ndi malangizo: Werengani ndikumvetsetsa Mapepala a Chitetezo (SDSs) operekedwa ndi mankhwalawo. wopanga musanasunge, kugwira, kapena kugwira ntchito ndi mankhwala aliwonse kapena zida zowopsa.
2.1 Chepetsani kukhudzana ndi mankhwala. Valani zida zodzitetezera zoyenera pogwira mankhwala (mwachitsanzo, magalasi otetezera chitetezo, magolovesi, kapena zovala zodzitetezera).
2.2 Chepetsani kukomoka kwa mankhwala. Osasiya zotengera mankhwala zotsegula. Gwiritsani ntchito kokha ndi mpweya wokwanira (mwachitsanzo, fume hood).
2.3 Yang'anani pafupipafupi ngati mankhwala akutuluka kapena kutayikira. Ngati kutayikira kapena kutayikira kumachitika, tsatirani njira zoyeretsera wopanga monga momwe akulimbikitsira mu SDS.
2.4 Phatikizani zinyalala zamakemiko mu hood ya fume.
2.5 Onetsetsani kuti mukugwiritsa ntchito zinyalala zoyambirira ndi zachiwiri. (Chinthu choyambirira
Chidebe chimasunga zinyalala zomwe zangochitika kumene. Chidebe chachiwiri chimakhala ndi zotayikira kapena kudontha kuchokera mu chidebe choyambirira. Zotengera zonse ziwirizi ziyenera kukhala zogwirizana ndi zinyalalazo ndikukwaniritsa zofunikira za federal, state, ndi zakomweko zosungirako ziwiya.).
2.6 Mukataya chidebe chotayira, sindikizani ndi kapu yomwe mwapereka.
2.7 Khazikitsani (mwa kusanthula ngati kuli kofunikira) zinyalala zomwe zimapangidwa ndi ntchito, ma reagents, ndi magawo omwe amagwiritsidwa ntchito mu labotale yanu.
2.8 Onetsetsani kuti zinyalala zasungidwa, kusamutsidwa, kunyamulidwa, ndi kutayidwa motsatira malamulo onse a m’deralo, chigawo/chigawo, ndi/kapena dziko.
2.9 Zida zotulutsa ma radiation kapena biohazardous zingafunike kugwiridwa mwapadera, ndipo zoletsa zotayika zitha kugwira ntchito.
3.Zachilengedwe ngozi chitetezo
Kupezeka kwa Biohazard. Kutengera ndi zitsanzo zomwe zimagwiritsidwa ntchito pachidachi, pamwamba pake zitha kuonedwa ngati biohazard. Gwiritsani ntchito njira zoyenera zochotseratu tizilombo toyambitsa matenda pamene mukugwira ntchito ndi biohazard.
Zithunzi za BIOHAZARD. Zitsanzo zachilengedwe monga minyewa, madzi amthupi, tizilombo toyambitsa matenda, ndi magazi a anthu ndi nyama zina zimatha kufalitsa matenda opatsirana. Tsatirani malamulo onse a m'deralo, chigawo, chigawo, ndi/kapena dziko. Valani zida zodzitchinjiriza zoyenera, zomwe zimaphatikizapo, koma sizimangokhala: zovala zodzitchinjiriza, zoteteza kumaso, zovala/malabu, ndi magolovesi. Ntchito zonse ziyenera kuchitidwa m'malo okhala ndi zida zoyenera pogwiritsa ntchito zida zoyenera zotetezera (mwachitsanzo, zida zosungira). Anthu akuyenera kuphunzitsidwa molingana ndi zofunikira zamakampani ndi mabungwe asanagwire ntchito ndi zida zomwe zitha kupatsirana.
Werengani ndikutsatira malangizo omwe akugwiritsidwa ntchito ndi/kapena zowongolera zotsatirazi:
Ku US: Malangizo a Dipatimenti ya Zaumoyo ndi Zaumoyo ku US zofalitsidwa mu Biosafety in Microbiological and Biomedical Laboratories zopezeka pa: www.cdc.gov/biosafety.
Miyezo ya Occupational Safety and Health Standards, Pathogens Bloodborne Pathogens (29 CFR§1910.1030), yopezeka pa:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Ma protocol a kampani yanu/bungwe la Biosafety Programme pogwira ntchito ndi/kugwira zinthu zomwe zitha kupatsirana. Zambiri zokhuza malangizo a biohazard zimapezeka pa: www.cdc.gov.
Mu EU: Yang'anani malangizo amdera lanu ndi malamulo okhudzana ndi chitetezo chachilengedwe komanso njira zabwino zotsatiridwa ndi World Health Organisation (WHO) Laboratory Biosafety Manual, kope lachitatu, lopezeka pa: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
Zogwirizana nazo
-
SARS-CoV-2 Antigen Assay Kit (Immunochromatogr...
SARS-CoV-2 Antigen Assay Kit (Immunochromatography Method) Buku la Zogulitsa 【PRODUCT NAME】SARS-CoV-2 Antigen Assay Kit (Njira ya Immunochromatography) [KUPANGITSA ZOCHITIKA] 1 Mayeso/Kit nonoyi ya β ACT ndi β ACT getavirus】 COVID-19 ndi matenda opatsirana pachimake. Nthawi zambiri anthu amavutika. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye omwe amatsogolera ...